Food safety is a global priority, and one of the leading causes of foodborne illness worldwide is Salmonella. This bacterium can contaminate a wide range of foods—meat, poultry, eggs, dairy, fruits, and vegetables—and is responsible for salmonellosis, a disease that affects millions of people every year. To reduce risks and ensure compliance with safety standards, rapid and accurate detection methods are essential. One of the most advanced and trusted techniques today is Polymerase Chain Reaction (PCR).